_3H-TdR掺入率对骨髓造血细胞低温保存效果的评价

ASSESSMENT OF ~3H-TdR INCORPORATION FOR THE VIABILITY OF BONE MARROW HEMATOPOIETIC STEM CELLS AFTER FREEZING AND THAWING

利用~3H-TdR掺入率液体闪烁测定法结合台盼蓝拒染率和单层琼脂CFU-GM培养对骨髓造血细胞低温保存后的活力进行了研究。观察了骨髓MNC的不同细胞浓度和孵育时间、不同保存温度(新鲜,4℃,-80℃,液氮)和保存时间(30分,7天,28天,90天)的~3H-TdR掺入年,并与台盼蓝拒染率、单层琼脂CFU-GM培养进行了对比,表明~3H-TdR掺入率是评价低温保存后骨髓造血细胞活力的一项敏感可靠的指标,周期短,并可较准确的反映细胞中处于DAN合成期(S期)的细胞含量。

We have studied ~3H-TdR incorporation rates of bone marrow MNC at different concentrations and incubation periods;also we compared with the viability of MNC preserved at different range of time and tempe- ratures by means of ~3H-TdR incorporation combined with Trypan Blue and CFU-GM culture methods.The result suggested that ~3H-TdR incorporation Was a sensitive and reliable assess to evaluate the viability of bone marrow MNC after cryopreservation and thawing.