摘要
为定量分析高胆固醇血症对动脉内皮细胞的损伤,并观察调脂通脉灵对此损伤可能存在的保护作用,采用内皮细胞铺片法和放射自显影技术,对鹌鹑在体动脉内皮细胞再生进行了定量分析。研究发现,高胆固醇组动脉内皮细胞排列不规则、密度增加,~3H-TdR标记率为2.05±4.85‰,与对照组相比(~3H-TdR标记率为0.81±0.87‰)差异有显著性。经调脂通脉灵治疗后,血脂浓度明显降低,~3H-TdR标记率随之下降。提示:通过对内皮细胞再生的定量分析,可对内皮细胞的损伤程度作出定量分析。
The aortic endothelial cell replication quails with hypercholes-
terolemia was quantitatively researched by special method of en-face prepa-
ration and ~3H-thymidine autoradiography.The endothelial cells arrange
disorderly with high density.The labelling rate of ~3H-TdR is 0.205±0.485%
per 24 hours in hypercholesterolemia group and significantly higher than
that(0.081±0.087% per 24 hours)in control group(P<0.01).The
cholesterol concentration was reduced by the treatment of TZTML(traditional
Chinese medicine)and the labelling rate was reduced too.The results indi-
cate that the endothelium injury can be quantitatively analyzed with the
index of ~3H-TdR labelling rate.
出处
《山东医科大学学报》
1993年第3期184-188,共5页
Acta Academiae Medicinae Shandong
基金
国家自然科学基金(3880395)
关键词
动脉
内皮细胞
损伤
高胆固醇血症
Hypercholesterolemia
Arteries
Endothelium cells
Wounds and injuries
Fixed quantity analyses
Quail
