子宫内膜癌中诱导型一氧化氮合酶表达及与肿瘤血管形成关系

Expression of Inducible Nitric Oxide Synthase in Endometrial Carcinoma and Its Relation to Carcinoangiogenesis

[目的]探讨诱导型一氧化氮合酶(induciblenitricoxidesynthase,iNOS)在子宫内膜癌组织中表达及与肿瘤血管形成的关系。[方法]采用免疫组化S-P法检测30例子宫内膜癌组织中iNOS和血管内皮生长因子(vascularendothelialgrowthfactor,VEGF)的表达和微血管密度(microvesseldensity,MVD)。[结果]iNOS在30例子宫内膜癌组织中的阳性表达率为73.3%;而对照组正常子宫内膜均未见表达。子宫肌层浸润深度>1/2的iNOS表达明显高于肌层未受累或≤1/2的患者(100%vs57.9%,P<0.05)。iNOS表达阳性组和阴性组MVD均值分别为45.6±3.5和20.8±4.5,两组比较差异有显著性(P<0.01)。iNOS的表达与VEGF及MVD均呈正相关(r=0.95,P<0.01;r=0.25,P<0.01)。[结论]iNOS在子宫内膜癌组织中的高表达与肿瘤血管形成及子宫肌层浸润程度的密切相关性,提示iNOS的高表达可能参与诱导肿瘤血管的形成。

To investigate the expression of inducible nitric oxide synthase (iNOS) in endometrial carcinoma and its relation to angiogenesis.The expression of iNOS,vascular endothelial growth factor(VEGF)and microvessel density (MVD) in 30 cases with endometrial carcinoma was evaluated by immunohistochemistry S-P staining.The positive expression rate of iNOS in 30 cases with endometrial carcinoma was 73.3%,but no expression in normal endometrium. The positive rate of iNOS was significantly higher in the cases with myometrium invasion with invasion depth over 1/2 than that in the cases with myometrium invasion less than 1/2(100% vs 57.9%,P<0.05).The value of MVD was 45.6±3.5 and 20.8±4.5 in positive and negative expression of iNOS respectively.There was significant difference between two groups(P<0.01).The expression of iNOS was positively correlated with expression of VEGF and MVD (r =0.95, P<0.01;r=0.25, P<0.01, respectively.[Conclusions]The high expression of iNOS in endometrium carcinoma is closely related to angiogenesis and myometrium invasion. It reveals that the high expression iNOS may participate in tumor angiogenesis.