应用基因表达系列分析方法进行宫颈上皮基因表达谱的研究

A serial analysis of gene expression (SAGE) in gene expression profile of cervical epithelium

目的 评价基因表达系列分析方法在宫颈鳞状上皮基因表达谱研究中的作用。方法 应用基因表达系列分析方法 (serialanalysisofgeneexpression ,SAGE .)建立正常宫颈鳞状上皮基因表达的SAGE文库。结果 经SAGE分析获得 13886个特异标签 ,从中得到 195 0个特异基因信息。结论 SAGE是一种能够快速、高通量地研究组织细胞基因表达谱的方法。

Objective To investigate the gene expression profile of cervical epithelium using a serial analysis of gene expression (SAGE).Methods The SAGE library of normal cervical squamous cells was constructed by a serial analysis of gene expression (SAGE).Results A total of 13 886 SAGE tags were analyzed,and 1 950 specific genes were revealed.Conclusions SAGE is a powerful method to investigate the gene expression profile,allowing the analysis of the expression of thousands of transcripts in a quantitative manner without prior sequence information.