非小细胞肺癌组织中hTERT、MDR1和MRPmRNA与C-myc蛋白的表达及相关性研究

Relationships among Expressions of hTERT, MDR1, MRP mRNA, and C-myc Protein in Non-small Cell Lung Cancer

背景与目的:已证实myc蛋白可上调某些肿瘤中人端粒酶逆转录酶(human telomerase reverse transcriptase,hTERT)、多药耐药基因(multidrug resistance gene,MDR1)、多药耐药相关蛋白(multidrug-resistance-related protein,MRP)mRNA的表达,hTERT与化疗疗效有关。阐明肺癌中myc、hTERT与多药耐药相关基因的内在关系,可为治疗提供更多思路。本研究旨在检测NSCLC中hTERT、MDR1、MRPmRNA以及C-myc蛋白的表达,探讨它们之间的相关性。方法:采用原位分子杂交及SP免疫组化方法检测NSCLC组织中hTERT、MDR1、MRPmRNA和C--myc蛋白的表达,并作统计学相关分析。结果:113例NSCLC中hTERT、MDR1、MRPmRNA和C-myc蛋白的阳性率分别为80.5%、51.3%、80.5%、68.1%。NSCLC组织类型等临床病理特征间四项指标阳性率无显著性差异(P值均>0.05)。hTERTmRNA与MDR1mRNA、MRPmRNA、C-myc蛋白三者相关性有统计学意义(P<0.05)。C-myc蛋白与MDR1mRNA、MRPmRNA无显著性相关(P>0.05)。结论:NSCLC中hTERTmRNA与MDR1mRNA、MRPmRNA及C鄄myc蛋白表达有关。C-myc的过度表达可能是hTERT的调节机制之一。

BACKGROUND & OBJECTIVE: The latest researches showed that myc protein could up-regulate the expression of human telomerase reverse transcriptase (hTERT), multidrug resistance gene 1(MDR1), multidrug resistance-related protein (MRP) in some kinds of tumors, and hTERT is correlated with efficiency of anti-tumor chemotherapy. This study was to investigate relations among expressions of hTERT, MDR1, MRP mRNA, and C-myc protein in non-small cell lung cancer (NSCLC). METHODS: Expressions of hTERT, MDR1, MRP mRNA in 113 cases of NSCLC tissues were detected by in situ hybridization, expression of C-myc protein was detected by SP immunohistochemistry, their correlations with clinicopathologic features of NSCLC were statistically analyzed. RESULTS: Positive rates of hTERT, MDR1, MRP mRNA, and C-myc protein in NSCLC tissues were 80.5%, 51.3%, 80.5% and 68.1%, repectively. Expressions of MDR1, MRP mRNA, and C-myc protein were significantly related to that of hTERT mRNA (P<0.05). Expression of C-myc protein did not correlate with expression of MDR1 or MRP mRNA. All 4 factors have no correlation with clinicopathologic features of NSCLC (P>0.05). CONCLUSION: Expression of hTERT mRNA may be related to those of MDR1, MRP mRNA, and C-myc in NSCLC. Overexpression of C-myc protein may be one of the molecular regulatory mechanisms of hTERT mRNA.