期刊文献+

可诱导共刺激分子在系统性红斑狼疮患者外周血T淋巴细胞的表达 被引量:4

Expression of Inducible Co-Stimulator on T lymphocytes in Peripheral Blood from the Patients with Systemic Lupus Erythematosus
原文传递
导出
摘要 目的探讨可诱导共刺激分子(ICOS)在系统性红斑狼疮(SLE)患者外周血T淋巴细胞的表达。方法应用双色荧光抗体染色技术经流式细胞仪检测了33例SLE患者和16例正常人对照者外周血中T淋巴细胞表面ICOS的自然表达水平,同时收集SLE患者的实验室检查指标,并用SLE疾病活动指数(SLEDAI)来判定SLE患者疾病活动程度,比较分析不同组别SLE患者ICOS表达水平与SLEDAI的相关性。结果活动期SLE组T淋巴细胞ICOS表达水平明显高于正常人对照组(P<0.01)和非活动期SLE组(P<0.01),非活动期SLE组与正常人对照组T淋巴细胞ICOS表达水平则差异无统计学意义(P>0.05)。活动期组与非活动期组SLE患者T淋巴细胞ICOS表达水平均与SLEDAI呈显著正相关(r=0.71,P=0.001、r=0.56,P=0.03)。结论活动期SLE患者T淋巴细胞ICOS表达增高,ICOS可能与SLE的发病机制有关。 Objective To investigate the expression of inducible co-stimulator (ICOS) on T lymphocytes in peripheral blood from the patients with systemic lupus erythematosus (SLE). Methods Two-color immunofluorescent staining and flow cytometric assay were used to analyze the level of ICOS expression on T lymphocytes in peripheral blood from 33 SLE patients and 16 healthy volunteers. The data of systemic lupus erythematosus diseases activity index (SLEDAI) in SLE patients were also collected. Results The level of ICOS expression on T cells from active SLE group was markedly higher than that in the control group and the inactive SLE group (P < 0.01). But there was no significant difference between the inactive SLE group and the control group (P > 0.05). However, a positive correlation between the expression level of ICOS and the SLEDAI score was found in both active SLE group and inactive SLE group(r = 0.711,P = 0.001?r = 0.561,P = 0.03). Conclusions The expression of ICOS on T cell in peripheral blood from active SLE is increased, which might play a role in the pathogenesis of SLE.
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2005年第1期8-10,共3页 Chinese Journal of Dermatology
关键词 ICOS 患者 SLEDAI T淋巴细胞 表达水平 活动期 可诱导共刺激分子 结论 组别 收集 Lupus erythematosus, systemic Molecule,inducible co-stimulatory T-lymphocytes Antigens, CD28
  • 引文网络
  • 相关文献

参考文献6

  • 1Criscione LG, Pisetsky DS. B lymphocytes and systemic lupus erythematosus. Curr Rheumatol Rep, 2003, 5: 264-269.
  • 2Seo SJ, Fields ML, Buckler JL, et al. The impact of T helper and Tregulatory cells on the regulation of anti-double-stranded DNA B cells. Immunity, 2002, 16: 535-546.
  • 3Hutloff A, Dittrich AM, Beier KC, et al. ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28. Nature,1999, 397: 263-266.
  • 4lwai H, Abe M, Hirose S, et al. Involvement of inducible costimulatot-B7 homologous protein costimulatory pathway in routine lupus nephritis. J Immunol, 2003, 171: 2848-2854.
  • 5Bombardier C, Gladman DD, Urowitz MB, et al. Derivation of the SLEDAI. A disease activity index for lupus patients. The Committee on Prognosis Studies in SLE. Arthritis Rheum, 1992, 35: 630-640.
  • 6Mages HW, Hutloff A, Heuck C, et al. Molecular cloning and characterization of murine ICOS and identification of B7h as ICOS ligand. Eur J Immunol, 2000, 2,0: 10d0-10d7.

同被引文献67

  • 1杨佳荟,沈茜,赵东宝,蔡青,韩星海.可诱导共刺激分子在系统性红斑狼疮病人外周血T细胞亚群的表达及与临床的关系[J].中华医学杂志,2005,85(5):318-323. 被引量:4
  • 2Hutloff A, Dittrich AM, Beier KC, et al. ICOS is an inducible T- cell co-stimulator structurally and functionally related to CD28. Nature, 1999, 397: 263-266.
  • 3Okamoto T, Saito S, Yamanaka H, et al. Expression and function of the co-simulator H4/ICOS on activated T cells of patients with rheumatoid arthritis. J Rhcumatol, 2003, 30:1157-1163.
  • 4Wiendl H, Neuhaus O, Mehling M, et al. The CD28 related molecule ICOS: T cell modulation in the presence and absence of B7.1/2 and regulational expression in multiple sclerosis. J Neuroimmunol, 2003, 140: 177-187.
  • 5Flesch IE. Inducible costimulator (ICOS). J Biol Regul Homeost Agents, 2002, 16:214-216.
  • 6Swallow MM, Wallin JJ, Sha WC, et al. B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFalpha. Immunity, 1999, 11: 423-432.
  • 7Khayyamian S, Hutloff A, Büchner K, et al. ICOS-ligand, expressed on human endothelial cells, costimulates Thl and Th2 cytokine secretion by memory CD4^+ T cells. Proc Natl Acad Sci USA, 2002, 99: 6198-6203.
  • 8Carreno BM, Collins M. The B7 family of ligands and its receptors: new pathways for costimulation and inhibition of immune responses. Annu Rev Immunol, 2002, 20: 29-53.
  • 9Mages HW, Hutloff A, Heuck C, et al. Molecular cloning and characterization of murine ICOS and identification of BTh as I- COS ligand. Eur J Immunol, 2000, 30: 1040-1047.
  • 10Beier KC, Hutloff A, Dittrich AM, et al. Induction, binding specificity and function of human ICOS. Eur J Immunol, 2000, 30: 3707-3717.

引证文献4

二级引证文献5

;
使用帮助 返回顶部