低温对诱导小麦未成熟胚愈伤组织出愈率的影响研究

Effect of low-temperature on the forming ratio of callus of wheat green embryo.

试验研究低温处理对诱导小麦未成熟胚愈伤组织出愈率结果表明 ,6h短时间低温处理可显著提高其出愈率 ,比对照增加 1 4 3倍。随处理时间的延长其出愈率逐渐降低 ,18h后其出愈率又渐增 ,低温处理 30h时其出愈率显著高于对照 ,为对照的 1 4 1倍。

The results show that the pretreatment in low -temperat ure for 6h can increase the callus formation ratio apparently,being 1.43 times h igher than that of the control;the callus forming ratio is decreased along with the prolongation of the low -temperature time,b ut the callus forming ratio increa ses after 18h low -temperature treatment.The pr etreatment of lo w -temperature for 30h can also increase the ca llus forming ratio by 1.41 times.