透明质酸介导的细胞游走受体和CD_(44v6)在膀胱癌侵袭转移中的作用

Role of Receptor for HA-Mediated Motility and CD_(44v6) in Invasion and Metastasis of Bladder Cancer

目的 探讨透明质酸介导的细胞游走受体 (RHAMM )、CD44v6在膀胱癌侵袭转移中的作用。方法 采用免疫组织化学检测膀胱癌RHAMM ,CD44v6的表达 ;Westernblot法检测膀胱癌RHAMM蛋白表达。结果 膀胱癌RHAMM、CD44v6阳性表达率分别为 60 .9%和 5 3 .1%。随着膀胱癌进展RHAMM阳性表达有增高趋势 ,而CD44v6表达显著下降 ,与病理分期、术后复发及转移有关(P <0 .0 5 )。Westernblot证实膜型RHAMM蛋白在膀胱癌有上调表达。结论 RHAMM、CD44v6异常表达与膀胱癌侵袭及预后有关 ,可能是判断膀胱癌恶性表型及预后的指标之一。

Objective To explore the role of receptor for HA mediated motility (RHAMM) and CD 44v6 in the invasion and metastasis of bladder carcinoma.Methods The expression of RHAMM and CD 44v6 in 64 cases of bladder carcinoma was detected by immunohistochemistry.The level of RHAMM protein in bladder carcinoma was estimated by Western blot.Results The positive expression rate of RHAMM,CD 44v6 in bladder carcinoma was 60.9% and 53.1%, respectively.With the invasion of bladder carcinoma,the expression of RHAMM was increased and was related to the pathological stages,postoperational recurrence and metastasis ( P < 0.05), while the expression of CD 44v6 decreased obviously and reversely related to the postoperational recurrence and metastasis ( P < 0.05). Western blot suggested that the membrane type RHAMM protein was up regulated in the bladder carcinoma with invasion and metastasis.Conclusion The abnormal expression of RHAMM and CD 44v6 was related to the invasion,metastasis and prognosis of bladder carcinoma,and may be one of the indexes for evaluating the malignant phenotype and prognosis of bladder carcinoma.