三氧化二砷对激素非依赖性前列腺癌PC-3细胞Caspase-3活性及Survivin基因表达的影响

Effect of arsenic trioxide on cell Caspase-3 activity and Survivin gene expression in hormone refractory prostate cancer PC-3 cell line

目的 探讨三氧化二砷对激素非依赖性前列腺癌PC 3细胞株细胞增殖、细胞凋亡、Caspase 3活性及Survivin基因表达的影响。方法 以 1、2、3、6、10 μmol/L的三氧化二砷作用于体外培养的PC 3细胞 ,48h后对细胞增殖、细胞凋亡、Caspase 3活性 ,SurvivinmRNA表达进行检测。结果 48h后各浓度的三氧化二砷均可抑制PC 3细胞增殖 ,3、6、10 μmol/L处理组可检测到细胞凋亡 ,凋亡率分别为 11.8%、12 .7%、2 9.6% (P <0 .0 0 1)。1、2、3、6、10 μmol/L三氧化二砷组Cas pase 3活性较对照组升高10 .5、2 7.5、3 1.5、3 4.5及 42倍。对照组及 1、2、3、6、10 μmol/L组的Sur vivin基因表达强度分别为 1.11± 0 .2 4、0 .99± 0 .0 6、0 .89± 0 .12、0 .78± 0 .0 4、0 .5 1± 0 .0 6、0 .4±0 .0 5 ,差异具有统计学意义意义 (P <0 .0 1)。结论 三氧化二砷可抑制PC 3细胞的增殖 ,诱导凋亡 ,与三氧化二砷的浓度相关 ,这一作用与三氧化二砷抑制PC 3细胞中Survivin基因表达及增加Caspase 3的活性有关。

Objective To investigate the effect of arsenic trioxide on cell proliferation,apoptosis,Caspase 3 activity and Survivin gene expression in hormone refractory prostate cancer PC 3 cell line.Methods PC 3 cells were exposed to different concentrations of arsenic trioxide for 48 h.Cell proliferation activity,apoptosis,Caspase 3 activity and Survivin mRNA were measured.Results After being incubated for 48 h,arsenic trioxide could inhibit PC 3 cell proliferation in all concentrations tested.The apoptosis rate were 11.8%, 12.7%, 29.6% respectively when the concentrations of arsenic trioxide exposed were 3,6,10 μmol/L ( P < 0.001). The Caspase 3 activity in experimental groups was significantly increased as compared with that in the control group.The intensity of Survivin gene expression in PC 3 cells (control,1,2,3,6 10 μmol/L groups) was 1.11± 0.24, 0.99± 0.06, 0.89± 0.12, 0.78± 0.04, 0.51± 0.06 and 0.4± 0.05 respectively,with the difference being statistically significant ( P < 0.01). Conclusion Arsenic trioxide could effectively inhibit cell proliferation and induce apoptosis in PC 3 cells by suppressing Surviving gene expression and increasing the Caspase 3 activity.