真皮生物模板对成纤维细胞生物学行为的影响

EFFECTS OF DERMAL TEMPLATE ON THE BIOLOGICAL BEHAVIORS OF FIBROBLASTS DURING WOUND HEALING

目的 研究真皮生物模板的应用对皮肤组织创伤修复过程中成纤维细胞 (fibroblast,FB)生物学行为的影响 ,探讨其在影响组织修复及减轻瘢痕形成中的作用机制。 方法 SD大鼠 12 0只 ,在背部造成 2 .5 cm× 2 .5 cm的全层皮肤组织缺损创面 ,随机分为 4个处理组 ,即开放创面组、全厚皮移植组、刃厚皮移植组、复合移植组 (无细胞真皮基质 +刃厚皮移植 ) ,分别于术后 1、2、4、6及 12周取材。通过免疫组织化学及图像分析技术检测组织标本中肌成纤维细胞 (myofibroblast,MFB)的标志蛋白—— α平滑肌肌动蛋白 (α smooth muscle actin,α- SMA)的表达以及纤维连接蛋白(fibronectin,FN)、整合素 α2 、β1 及转化生长因子 β1 (transforming growth factorβ1 ,TGF- β1 ) )的表达量。 结果 复合移植组 α- SMA在术后 1～ 12周低于开放创面组 ,术后 1、4、6周低于刃厚皮移植组 ,术后 2、12周高于全厚皮移植组(P<0 .0 5 ) ;复合移植组 FN在术后 1～ 12周低于开放创面组和刃厚皮移植组 ,术后 1～ 4周高于全厚皮移植组 (P<0 .0 5 ) ;复合移植组整合素 α2 在术后 1～ 12周低于开放创面移植组 ,术后 1～ 4周低于刃厚皮移植组 ,术后 1、2周高于全厚皮移植组 (P<0 .0 5 ) ;复合移植组整合素 β1 在术后 1～

Objective To study the effects of dermal template on the biological behaviors of fibroblasts during wound healing. Methods A total of 120 rats were made full thickness wound modes on the dorsum and divided into 4 groups,in group 1, the wounds were allowed to heal by contraction(ConT);in group2, the wounds covered with full thickness skin grafts( FTSG); in group 3, the wounds were with split thickness skin grafts (STSG); and in group 4, the wounds were covered by dermal regeneration template with overlying thin split thickness autograft (ADMT).The specimens were obtained at one week, two weeks, four weeks, six weeks,and twelve weeks respectively. The expressions of α smooth muscle actin(α SMA,characteristic of MFB),fibronectin(FN), integrin α 2,β 1 and transforming growth factor β 1(TGF β 1) were examined by immunohistochemical analysis. Results Positive expression of α SMA、 FN、integrin α 2β 1 and TGF β 1 in ADMT groups was significantly lower than that in STSG group and ConT group, but higher than that in FTSG group( P< 0.05). Conclusion Dermal regeneration template can inhibit the transformation of FB to MFB and restrain the expression of FN,integrin α 2,β 1,and TGF β 1 in fibroblasts which might reduce the possibility of hypertrophyic scaring, and improve wound healing.