p53基因第72位密码子多态与瘢痕疙瘩易感性研究

p53 GENE CODON 72 POLYMORPHISM AND SUSCEPTIBILITY TO KELOID IN CHINESE POPULATION

目的 探讨 p5 3基因第 72位密码子多态与瘢痕疙瘩遗传易感性的关系。 方法 采用聚合酶链反应 -反向斑点杂交、DNA直接测序方法 ,检测了 15例瘢痕疙瘩患者与 15名正常人对照的 p5 3基因第 72位密码子的基因型。 结果 瘢痕疙瘩组的 Pro等位基因及 Pro/ Pro基因型频率明显高于对照组 (P值分别为 0 .0 35、0 .0 30 ) ,Pro/Arg、Arg/ Arg基因型频率在病例组和对照组的分布差异无显著性。 结论 p5 3基因第 72位密码子 Pro等位基因及Pro/ Pro基因型可能是瘢痕疙瘩的易感因素。

Objective To investigate the relationship between p53 codon 72 polymorphism and susceptibility to keloid. Methods The p53 genotypes were detected by polymerase chain reaction reverse dot blot(PCR RDB) and DNA direct sequencing among 15 healthy controls and 15 patients with keloid. Results The frequency of the Proallele( P =0.035) and Pro/Pro genotype(P=0.030) in patients was significantly higher than that in the controlls. There was no significant difference in the frequency of Pro/Arg and Arg/Arg genotypes between patients and controls. Conclusion The p53 gene codon 72 polymorphism may play a role in susceptibility to keloid.