病理性瘢痕中热休克蛋白47的表达与胶原沉积相关性研究

STUDY OF THE EXPRESSION OF HEAT SHOCK PROTEIN 47 AND ITS CORRELATION TO COLLAGEN DEPOSITION IN PATHOLOGICAL SCAR TISSUES

目的 探讨热休克蛋白 4 7(heatshock protein 4 7,HSP4 7)在病理性瘢痕中的表达与胶原沉积的相关性。 方法 取经病理科确诊的正常皮肤 (10名 )、增生性瘢痕 (19例 )和瘢痕疙瘩 (16例 )组织标本 ,应用免疫组织化学方法、苦味酸 -天狼星红偏振光分析法检测组织中 HSP4 7的表达及胶原纤维含量。 结果 增生性瘢痕平均 IOD值5 2 115 9.5 0± 2 72 994 .13,瘢痕疙瘩组织平均 IOD值 4 0 74 4 0 .30± 2 95 780 .6 3中 HSP4 7表达量显著高于正常皮肤组织平均 IOD值 130 5 0 .17± 4 789.4 1,差异有统计学意义 (P<0 .0 1) ;HSP4 7的表达量与病理性瘢痕总胶原纤维含量呈正相关关系 (r=0 .386 ,P<0 .0 5 )。 结论 HSP4 7在病理性瘢痕中异常高表达 ,并可能在病理性瘢痕胶原沉积的过程中发挥重要作用。

Objective To study the expression of heat shock protein 47 (HSP47) and its correlation to collagen deposition in pathological scar tissues. Methods The tissues of normal skin(10 cases), hypertrophic scar(19 cases), and keloid(16 cases) were obtained. The expression of HSP47 was detected by immunohistochemistry method. The collagen fiber content was detected by Sirius red staining and polarization microscopy method. Results Compared with normal skin tissues(Mean IOD 13 050.17 ±4 789.41), the expression of HSP47 in hypertrophic scar(Mean IOD 521 159.50 ± 272 994.13) and keloid tissues(Mean IOD 407 440.30 ± 295 780.63) was significantly high( P <0.01). And there was a direct correlation between the expression of HSP47 and the total collagen fiber content( r =0.386, P <0.05). Conclusion The HSP47 is highly expressed in pathological scar tissues and it may play an important role in the collagen deposition of pathological scar tissues.