摘要
目的观察补阳还五汤对大鼠脑缺血-再灌注损伤的保护作用。方法SD大鼠56只随机分假手术组、模型对照组、补阳还五汤1组和补阳还五汤2组,每组14只。线栓法制作大鼠脑缺血-再灌注模型。再灌注48小时后观察大鼠神经功能缺损评分情况,TTC染色测量脑梗死体积,干湿重法测量缺血侧脑含水量,常规HE染色观察病理变化。结果脑缺血-再灌注48小时,补阳还五汤1、2组大鼠神经功能缺损评分明显高于对照组(P<0.05),脑梗死体积及脑水肿也明显减轻(P均<0.05),脑组织病理学显著改善。补阳还五汤1、2两组比较,2组疗效稍好于1组,但无统计学意义(P>0.05)。结论补阳还五汤对大鼠脑缺血-再灌注损伤具有保护作用。
Objective: To observe the effect of Buyang Huanwu Decoction on cerebral ischemia and reperfusion injuries in rats. Methods: 56 normal male SD rats were distributed randomly to 4 groups: ①sham-operation (P,n=14) ②control (C,n=14) ③Buyang Huanwu Decoction 1(B_1,n=14) ④Buyang Huanwu Decoction (B_2,n=14). Cerebral ischemia and reperfusion rat models were made using the method of thread inserting right middle cerebral artery occlusion (MCAO). The change of neurologic deficits, infarct volume and brain water content were measured 48 hours after reperfusion. At the same time, pathological change was observed. Results: Buyang Huanwu Decoction 1 and 2 groups can decrease the neurologyic deficits (P<0.05), relieve cerebral infarction volume (P<0.05), (subtract brainedema P<0.05), ameliorate the pathology. Constrasted Buyang Huanwu Decoction 1 with Buyang Huanwu Decoction 2, the effect of Buyang Huanwu Decoction 2 is better than Buyang Huanwu Decoction 1. But there is no statistic significance. Conclusion: All the results display the protective effects of Buyang Huanwu Decoction on cerebral ischemia and reperfusion injuries in rats.
出处
《浙江中西医结合杂志》
2005年第1期4-7,共4页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
