蝉拟青霉总多糖对老龄大鼠组织器官免疫功能调节的实验研究

Experimental study on the regulative effect of paecilomyces total polysaccharides on immunologic function in old rats

目的观察蝉拟青霉总多糖对老龄大鼠肝、肾、脾、胸腺等组织器官免疫功能的影响,探讨其对老龄大鼠的免疫调节作用。方法分别以双试剂终点法、速率法在全自动生化分析仪上测定酸性磷酸酶(ACP)及乳酸脱氢酶(LDH)活力;以鸟氨酸的生成判断精氨酸酶(Arg)的活力;以Ellman试剂测定还原型谷胱甘肽(GSH)水平;以硫代巴比妥酸醋酸法测定脂质过氧化物(LPO)含量。结果老龄大鼠ACP、LDH(肝、肾、脾)、Arg(肝、肾、胸腺)活力、GSH(肝、肾)水平显著上升,同时LPO(肝、肾)的含量下降。结论蝉拟青霉总多糖对老龄大鼠组织器官的免疫功能具有增强作用。

Objective To study the effect of paecilomyces cicadidae total polysaccharides on the immune regulative function of the liver, kidney, spleen and thymus in old rats. Methods The activities of lactate dehydrogenase (LDH), acid phosphatase (ACP) and arginase (Arg) were detected by automatic biochemistry analyzer. The reduced glutathione (GSH) level and the lipid peroxide (LPO) content were assayed. Results Compared with control group, the activities of ACP, LDH (liver, kidney, spleen) and Arg (liver, kidney, thymus), the level of GSH (liver and kidney) were significantly increased, and the LPO content was declined in the liver and kidney markedly. Conclusions Paecilomyces cicadidae total polysaccharides can promote immunologic function of tissues and organs in old rats.