黄芪注射液增强树突细胞的抗肺癌作用

Effects of Dendritic-cells Combined with Astragalus on Lewis Lung Cancer

目的 :研究黄芪注射液联合MHCI类限制性肿瘤抗原多肽Mut1致敏的树突细胞 (DCs)对肺癌小鼠的治疗作用及其免疫学原理。方法 :制备小鼠骨髓来源的树突细胞 ,用转移性Lewis肺癌特异性多肽Mut1预激DCs并联合黄芪注射液治疗肺癌小鼠 ,通过FACS分析其脾细胞内T淋巴细胞比例的变化 ,用Elisa法检测荷瘤小鼠血清内IL 2、IL 4浓度的变化 ,间接提示Th1 Th2比例的变化。同时免疫正常小鼠 ,观察宿主对随后肿瘤细胞攻击的保护作用。结果 :肿瘤抗原多肽致敏的DCs与黄芪注射液联合治疗后 ,比单用DCs更有效的治疗转移性肺癌 ,小鼠脾细胞内CD4 + T和CD8+ T细胞明显比例升高 ,联合治疗组的IL 2 IL 4比例也明显升高。在观察时间内经黄芪注射液和DCs联合免疫的小鼠成瘤率低于对照组和单用DCs组。结论 :以肿瘤抗原多肽致敏的DCs与黄芪注射液联合治疗能更有效的促进荷瘤宿主的免疫应答 ,具有显著的体内抑制肺癌转移的效果。对正常动物免疫保护作用更加明显。

Objective:To invetigate the antitumor effects and mechanisms of tumor antigen peptide pulsed dendritic cells combined with Astragalus.Methods:High purity DCs were obtained from 9 day culture of murine bone marrow cells.Mut1 is a MHC class I restricted tumor antigen peptide of Lewis lung cancer.DCs were incubated with Mut1,then vaccines were established.Lung cancer were established on the mice.The tumor bearing mice were treated and the survival period of the mice was observed.Treatment groups were divided into PBS,DC Mut1,DC Mut1 with Astragalus(DC Mut1+AG).The phenotypes of splenocytes of these treated mice were detected by FACS.Interleukin 2 and interleukin 4 in the serum were measured by cytokine specific ELISA.Normal mice developed a potent immune protection against the attack from tumor cells.The treatment groups were same as the previous groups.Results:The DC Mut1+AG group demonstrated antitumor effects with the regression majority of tumors.The proportion of the CD4 +T and CD8 +T cells in DC Mut1+AG group increased compared with that of DC Mut1 group( P <0 05).The IL 2 level of DC Mut1+AG group was higher than those of DC Mut1 group.The IL 4 level of DC Mut1+AG group was lower than that of the PBS group.Upon undergoing attack from LLC cells,DC Mut1+AG group had no formation of tumors.In contrast,4 of all the mice(6) treated with DC Mut1 developed tumors.Conclusion:DC Mut1+AG could exert antitumor effects in the mice and significantly inhibit tumor metastasis,which demonstrated a specific immune protection.