纤溶酶原激活物在肺血栓栓塞症发病机制中的作用

Study on Role of the Plasminogen Activator in the Pathogenesis of Acute Pulmonary Thromboembolism

目的:探讨纤溶酶原激活物(t鄄PA)在急性肺血栓栓塞症(PTE)发病机制中的作用。方法:应用酶联免疫吸附双抗体夹心法(ELISA法)定量测定了44例PTE患者和56例正常对照者血浆t鄄PA含量,并用发色底物法测定了血浆t鄄PA活性。结果:PTE组的t鄄PA含量和活性分别为33.88(20.91～48.74)μg/mL和(0.31±0.15)AU/mL,对照组分别为11.05(33.88～48.74)μg/mL和(0.40±0.21)AU/mL,差别均有统计学意义(P<0.05);吸烟、高血压和高甘油三酯PTE患者的t鄄PA活性显著减低。结论:多项临床、生化因素都可对t鄄PA活性产生重要影响;PTE患者的t鄄PA活性降低,含量升高。

Objective: To reveal the relationships of plasminogen activator antigen and activity levels to the incidence of PTE in Chinese Han ethnic group. To provide an objective evidence in the prophylaxis and management of PTE. Methods: The t-PA activity assay was measured by chromophorous substrate method and the antigen of t-PA was quantified by a commercially available enzyme-linked immunosorbent assay (ELISA) respectively in 44 PTE cases and 56 health control cases. Results: Comparing with health-control group, PTE patients had lower levels of t-PA activity[(0.31±0.15) AU/mL vs (0.40±0.21) AU/mL], but higher levels of t-PA antigen [33.88 (20.91-48.74) 靏/L vs 11.05(33.88-48.74) 靏/L]. In the patients with PTE, the levels of t-PA activity were influenced by smoking, blood pressure and TG. Conclusion: The decrease of t-PA activity levels in circulation probably is a risk factor for PTE, which is not caused directly by the defect of release or production of t-PA antigen. Several clinic and biochemistry factors could influence the levels of t-PA activity.