肺耐药相关蛋白基因和多药耐药基因测定在急性淋巴细胞白血病中的意义

The significance of lung drug resistance related protein gene(LDRP) and multi drug resistance gene (MDR-1) expression in acute lymphoblastic leukemia

目的:评价肺耐药相关蛋白基因和多药耐药基因在急性淋巴细胞白血病患者中的表达情况与临床多药耐药的关系。方法:将47例急性淋巴细胞白血病患者分为临床非耐药组(A)和耐药组(B),用RT-PCR方法检测患者骨髓中肺耐药相关蛋白基因和多药耐药基因的mRNA表达。结果:肺耐药相关蛋白基因在A、B组阳性表达分别占6/27和8/20。统计学分析显示组间差异无显著性意义(χ2=1.15,P>0.05)。多药耐药基因在A、B组阳性表达分别占3/27和9/20。统计学分析显示组间差异有显著性意义(χ2=2.98,P<0.05)。在所有20例临床耐药患者中,5例同时表达上述两个基因。而临床非耐药组患者中,无一例同时表达上述两个基本基因。结论:肺耐药相关蛋白基因不能作为独立判定急性淋巴细胞白血病多药耐药的发生指标,多药耐药基因在判定急性淋巴细胞白血病多药耐药的发生中有一定临床意义。

Objective To evaluate the relationship between lung drug resistance related protein (LDRP) gene and multi drug resistance gene (MDR 1) in acute lymphoblastic leukemia(ALL). Methods Forty seven patients were divided into non resistance group (A) and resistance group (B). The expression of LDRP and MDR 1 mRNA was detected by RT PCR. Results The positive expression of LDRP was 6/27 and 8/20 in A and B group, respectively. There was no significantly statistic difference of the expression between A and B group. The positive expression of MDR 1 was 3/27 and 9/20 in A and B group respectively, which in A group was lower than that in B group(χ2=2 98,P< 0.05). In the all 20 multi drug resistance patients, there were five persons who expressed both LDRP and MDR 1. However, there was no patient who expressed both genes in A group. Conclusion LDRP gene cant be regarded as a single indicator for multi drug resistance in ALL, while MDR 1 gene might be a good sign of it.