鸭乙型肝炎表面抗原硝酸醛复红染色的研究

OBSERVATION ON DHBsAg IN LIVER TISSUE BY NITRATED ALDEHYDE FUCHSIN STAINING IN THE DUCK

应用硝酸醛复红法观察了鸭肝组织的DHB_sAg及肝组织病变,结果是,1.DHB_sAg要对硝酸醛复红着色迅速,着色后DHB_sAg呈紫红色,背景为蓝绿色。2.DHB_sAg在肝细胞内的存在形式为(1)胞浆型,包括均浆型和包涵体型。(2)细胞膜型。(3)胞浆混合型,即均浆型和包涵体型混合存在。其中胞浆型占优势,细胞膜型少见。这与人类HB_sAg的表现形式及分布状态近似。3.鸭肝组织内DHB_sAg的存在与鸭肝病变关系不大。4.硝酸醛复红着色原理可能与DHB_sAg分子多肽内的二硫键被硝酸氧化有关。5.与斑点分子杂交法比较,灵敏度较差,阳性率为22.1%。

The purpose of this study is to explore a method by which an improved aldehyde fuchsin staining can be applied to formalin-fixed paraffin sections to den or strate DHBsAg in the duck. The results showed that 23 of 104 liver specimens had DHBsAg positive substance.The positive rate was 22.1%.The stained DHBsAg was purplish- red and the background in blue-green.The shate and distribution of DHBsAg in duck hepatic tissue were the same as the HBsAg in human hepatic tissue. The Cytoplasm type had much higher percentage which occupied nearly 70% of all the DHBsAg positive hepatic tissue.However,the cell membrane type had lower percentage. This method of DHBsAg staining with nitrated aldehyde fuchsin would be useful in the pathologic diagnosis of the duck hepatitis B infection。