大蒜新素对HCMV主要即刻早期抗原IE72和IE86在人胚肺成纤维细胞中表达的影响

Effects of allitridin on the expression of human cytomegalovirus immediate early antigens-IE72 and IE86 in human embryonic lung cells

目的 :研究大蒜新素对人巨细胞病毒 (HCMV)即刻早期抗原 (IEAs)在人胚肺成纤维细胞中表达的影响。方法 :建立HCMVAD16 9毒株 (MOI为 2.5和 0.2 5 )感染细胞模型和大蒜新素 (IC50 和MTC浓度 )干预模型。用WesternBlot检测药物处理后感染细胞中HCMV主要IEAs(IE72和IE86 )表达强度变化 ,并与相应浓度更昔洛韦(GCV)的作用效应相比较。结果 :在高MOI和低MOI感染时 ,不同浓度大蒜新素均能使HCMVIE72和IE86表达强度明显下降 ,对IE86表达的抑制率为IE72的 2倍左右。与GCV相比 ,大蒜新素对IE72的抑制率低于GCV ,而对IE86的抑制效应高于GCV。结论 :大蒜新素明显抑制HCMVIE72和IE86表达 ,对IE86的抑制尤为显著 。

Objective: To investigate the effect of allitridin injection on the expression of human cytomegalovirus (HCMV) immediate-early antigens(IEAs including IE72 and IE86)in human embryonic lung cells. Method: HCMV AD 169 Virus strain infected cell model (MOI=2.5 and 0.25,respectively) were established, and then treated with IC_(50) and MTC doses of allitridin. Western blot was used to analyze the changes of IE72 and IE86 expression after the treatment, ganciclovir(GCV, IC_(50) and 2.3×IC_(50)) treatment as control. Result: No matter what kind of MOI was used, both IE86 and IE72 antigens' expression was effectively suppressed by allitridin treatment, and the inhibitory rate of IE86 was almost twice of IE72′s. Compared with GCV, allitridin had stronger inhibitory effect on IE86 expressing, although its efficacy on IE72 was weaker than GCV. Conclusion: Allitridin could suppress the expression of IE72 and IE86, especially for IE86 expressing, maybe it is ore of key role in the mechanism of allitridin against HCMV.