摘要
目的探讨用打孔法测定克拉霉素片含量的方法。方法采用打孔器在底层培养基上打孔,用微量注射器加样后于37℃培养,测量抑菌圈直径,计算含量并与管碟法进行比较。结果打孔法测定在10~120IU·ml-1范围内有较好的线性关系。本法测定结果精密、准确,平均可信限率为2l%,低于管碟法(82%),平均回收率为99.8%。结论该法可代替管碟法测定克拉霉素片含量。
Objective Studies on the digging holes method for quantitative determination of Clarithromycin tablets Methods At the culture medium, dig holes by a corneal circle drill and inject a little liquid by a very small volume syinge,and than cultured in 37 ℃ ,survey diameter of the bacterostatic circle,reckon the content and compare with the method of pipe and dish Results The method of dig holes is linear within the range of 1 0~12 0 IU·ml -1 The average rate of the believable range is 2 1%, below the method of pipe and dish (8 2%) The average recovery is 99 8% Concclusion This method may instead of the method of the pipe and dish for quantitative determination of Clarithromycin tablets
出处
《江苏药学与临床研究》
2004年第6期67-69,共3页
Jiangsu Pharmacertical and Clinical Research
关键词
克拉霉素
打孔法
管碟法
Clarithromycin
Method of dig holes
Method of pipe and dish