重组葡激酶的致突变试验

Study on the Matagenesis of r-Sak

为观察重组葡激酶(r-Sak)能否诱发基因和染色体的改变而具有致突变作用,进行了哺乳动物培养细胞染色体畸变试验、啮齿动物微核试验和诱发鼠伤寒沙门氏菌回复突变试验。结果表明重组葡激酶浓度为0.128、0.64和3.2ug/ml时未发现CHL细胞明显的染色体损伤,畸变率在5％以下。NIH小鼠静注重组葡激酶的剂量为16.8与4.2mg/kg时微核发生率在5％以下,与空白对照组比较,无显著差异(P>0.05)。重组葡激酶(r-Sak)在1000、500、100、10、1ug/皿浓度时回变菌落数均在阴性对照组的两倍菌落数之内,不能诱发伤寒沙门氏菌回复突变。结果说明重组葡激酶无致突变作用。

The chromosome aberration of mammal cells, rnicronucleus test of rodent and the Ames test were investigated to observe whether r-Sak would cause mutation of gene or chromosome or have the action of mutagenesis. The results show that there were no chromosome damages significantly of CHL cell with the concentration of r-Sak 0. 128, 0.64, and 3.2ug/ml respectively, and the ratios of aberration were below 5% . with the desoge of r-Sak being 16, 8, 4, 2mg/kg respectively to NIH mouse by intravenous injection, The ratios of micronucleus cells were below 5%, and there was no signifecant difference from normal control (P>0. 05). the bacterial colony of back mutation with the r-Sak consentration 1000, 500, 100, 10, lug/ml were with in double times of negative control's. Conclusions: r-Sak can't cause back mutation of Salmonella typhi. It indicated that the r-sak had no action of metagenesis.