酯酶标记白血病外周血淋巴细胞的临床应用价值

THE CLINICAL VALUE OF PERIPHERAL LYMPHOCYTES MARKED BY ANAE IN LEU KEMIA PATIENTS

本文用酸性非特异性酯酶(ANAE)标记T淋巴细胞的方法,对白血病、MDS不同时期(未缓解期、缓解期或经治明显好转期)外周血T淋巴细胞(TL)及其亚群辅助性T淋巴细胞(Th)和抑制性T淋巴细胞(Ts)进行了对比和动态观察。结果显示,在病情未缓解时TL、Th、Th/TS比值均明显低于正常对照组(p<0.01),Ts高于对照组;经有效治疗后,随着病情好转/TL、Th、Th/Ts比值也逐步升高,至完全缓解后恢复正常;若治疗无效,TL、Th、Th/Ts比值也始终处于减低状态。因此,我们初步认为,用ANAE标记外周血T淋巴细胞的方法来观察TL、Th、Ts、Th/Ts比值的变化,对预测疗效,判断预后有一定意义,可作为临床观察疗效的一个指标。另外,通过淋巴细胞ANAE的检测来区别T、B淋巴细胞,对协助诊断慢性淋巴细胞白血病有一定价值。

By using the methed of T lymphocytes marked by ANAE (acid α-naphthyl acetate es- terase), we compared and observed peripheral T lymphocytes (TL) and its subgroup Th and Ts in leukemia and MDS of different stages. The result shows that in the unrelieved stage, TL, Th and Th/Ts are significantly lower than normal controls (p<0. 01),but Ts is higher than normal controls. After the effective therapy, TL, Th and Th/Ts increase gradually, and can get normal when in complete remission. If the treatment is invalid, TL, Th and Th/ Ts keep low. Therefore, we think that it is valuble in reckoning the curative effect and prog- nosis to use ANAE marked T lymphocytes. Moreover, it is helpful in the diagnosis of chron- ic lymphocytic leukemia by using ANAE to differentiate TL from BL (B lymhocyte).