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烟叶中FⅠ蛋白的简捷提取技术及其氨基酸成分分析 被引量:25

The extraction of fraction Ⅰ protein from tobacco leaves and its amino acid composition analysis
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摘要 经过多次摸索和探讨 ,找出了一条简捷提取烟叶中FI蛋白的技术方法。此方法每kg鲜烟叶可提取高纯度的FⅠ蛋白 5 .12g ,同时还可获得FⅡ蛋白 6.2 7g。FⅠ蛋白氨基酸成分分析的结果表明 ,它具有完全氨基酸 ,其必需氨基酸含量高于世界粮农组织 (FAO) The key to exploitation and utilization of fraction Ⅰ protein (FⅠP) is the technique of extracting FⅠP from tobacco leaves. The method of simple and large extraction of FⅠP was found after studying and exploring many times. With the method, 5.12g FⅠP of high purity and 6.27g FⅡP can be obtained from every 1000g fresh leaves. The amino acid composition of FⅠP was analyzed. Results showed that FⅠP contained complete amino acids, the average essential amino acid contents were much higher than that of the FAO reference protein standard. Besides, according to homogenized leaf curing (HLC) process, the tobacco leaves from which FⅠP and FⅡP were extracted have a lower protein content, and can be used as a material for producing safer cigarettes.
出处 《中国烟草学报》 EI CAS CSCD 2000年第2期16-20,共5页 Acta Tabacaria Sinica
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