反胶束体系的组成对胆甾醇酯酶催化水解维生素E醋酸酯活性的影响

Effect of composition of reversed micelle systems on the activity of cholesterol esterase catalyzing the hydrolysis of vitamin E acetate

目的考察反胶束体系的组成对胆甾醇酯酶催化水解维生素E醋酸酯活性的影响。方法以卵磷脂、聚乙二醇对辛基苯基醚为表面活性剂 ,以溶有维生素E醋酸酯的不同有机溶剂为油相制备不同反胶束溶液 ,将胆甾醇酯酶溶入制得的反胶束体系使维生素E醋酸酯水解 ,采用HPLC法测定水解产物维生素E的生成量。结果有机溶剂、体系含水量、表面活性剂和助表面活性剂及其配比对反胶束体系中酶的活性影响较大。结论 14mmol·L-1卵磷脂 6mmol·L-1胆固醇 环已烷反胶束体系 (W0 =10 5 )

Objective To investigate the effects of composition of reverse micelle systems on the activity of cholesterol esterase which catalyzes the hydrolysis of vitamin E acetate (VEA) in reverse micelle systems.Methods Cholesterol esterase was incorporated into reverse micelles in different organic solvents with surfactants lecithin and Triton X-100 and water to catalyze the hydrolysis of VEA which was dissolved into the oil phase of the reverse micelle solutions.The yield of α-tocopherol which was the hydrolysis product of VEA in various reaction systems was determined by HPLC.Results Organic solvent,water content,surfactant,co-surfactant,and their ratio have great influence on the catalysis activity of the enzyme in reverse micelle systems.Conclusions The reversed micelle system that consists of 14 mmol·L -1 lecithin and 6 mmol·L -1 cholesterol-cyclohexane is optimal reactive medium for the hydrolysis of vitamin E acetate catalyzed by cholesterol esterase in the systems investigated here.