重组病毒巨噬细胞炎性蛋白致畸胎研究及骨髓微核实验

Teratogenic effects and micronucleus test of recombinent viral macrophage inflammatory protein

目的:研究重组病毒巨噬细胞炎性蛋白(rvMIP)的致畸胎和致细胞染色体损伤作用。方法:采用大鼠致畸胎、小鼠骨髓微核实验。结果:不同浓度的rvMIP实验组SD大鼠母鼠的畸胎率分别为29%,17%和25%,胎鼠总畸形率分别为3.1%,2.5%和1.6%。NIH小鼠骨髓微核率在给药后24h雌鼠分别为1.50,1.00和1.00,嗜多染红细胞与正染红细胞比值(PCE/NCE)分别为0.4,0.4和0.5,雄鼠分别为1.33,1.33和1.67,PCE/NCE比值分别为0.6,0.5和0.5。给药后48h的微核率雌鼠分别为2.00,1.33和1.33,PCE/NCE比值分别为0.5,0.5和0.5,雄鼠分别为1.50,2.00和1.00,PCE/NCE比值分别为0.3,0.4和0.5。各项检测指标在实验组与阴性对照组之间差别没有显著意义,P>0.05;在实验组与阳性对照组之间差别有非常显著意义,P<0.01。结论:在本实验条件下,rvMIP没有致畸胎和细胞染色体损伤作用。

AIM: To explore the effects of viral macrophage inflammatory protein(rvMIP) on teratogenicity and the rate of micronucleus. METHODS: The tests of teratogenesis in rates and micronucleus analysis in bone marrow cells of mice were adopted. RESULTS: The abnormal embryo rates induced by different concentration rvMIP on SD maternal rats were 29 %, 17 % and 25 %, respectively. The overall teratosis rates were 3.1 %, 2.5 % and 1.6 %, respectively the rate of micronucleus in NIH female mice induced by different concentration rvMIP 24 hours after intravenous administrotion were 1.50, 1.00 and 1.00, those in male mice were 1.33, 1.33 and 1.67, polychromatie erythrocytes/normochromatic erythrocytes (PCE/NCE) were 0.4, 0.4 and 0.5, and those in male mice were 0.6, 0.5 and 0.5. The rate of micronucleus in NIH femal mice induced by different concentration rvMIP 48 hours after intravenous administration were 2.00, 1.33 and 1.33, those in male mice were 1.50, 2.00 and 1.00, PCE/NCE were 0.5, 0.5 and 0.5, and those in male mice were 0.3, 0.4 and 0.5. Each parameter had no significant difference as compared with the negative control group (P>0.05), but had obvious difference between tested groups and positive group (P<0.01). CONCLUSION: rvMIP did not exert any effect on embryotoxicity and chromosome damage.