牙周病和龋病致病菌的共聚及共聚抑制

Coaggregation and coaggregation inhibition between perio-pathogenic and cariogenic bacteria

目的 :研究牙周病和龋病致病菌的共聚和共聚抑制关系及特征 ,筛选有效共聚抑制剂。方法 :以 4株牙周致病菌 (具核梭杆菌、伴放线放线杆菌、牙龈卟啉单胞菌和中间普氏菌 )和 4株致龋菌 (变形链球菌、血链球菌、粘性放线菌和嗜酸乳杆菌 )的国际标准菌株为研究对象 ,通过目测法和比浊法确定各组合细菌的共聚度并研究乳糖和精氨酸对共聚度 >+ 2 (或 >2 0 % )共聚对的共聚抑制作用。结果 :Fn Av、Pg Av、Fn Sm、Fn Ss、Fn La和Pg Ss共聚度 >2 0 % ;4.5～ 6.0mM精氨酸对除Fn Av外 5对有明显抑制作用 ,解聚度为 49%～ 92 % ;12 0～ 3 0 0mM乳糖仅对上述 6对共聚对中的Pg Ss有明显抑制作用 ,解聚度为 5 7%～ 91% ,对Pg Av有部分抑制作用 ,最大解聚度约为 3 5 % ,但对Fn G+ 菌几乎无效。结论 :牙周致病菌和致龋菌之间存在高度特异的共聚反应 ,精氨酸为有效共聚抑制剂。

Objective:To screen the coaggragating pairs between perio-pathogenic and cariogenic bacteria and to investigate the susceptibility of these coaggregations to inhibitors.Method:4 strains of perio-pathogenic bacteria,Fusobacterium nuleatum(Fn)ATCC 10953, Actinobacilllus actinomycetem comitans (Aa)Y4, Porphyromonas gingivalis(Pg )ATCC 33277 and Prevotella intermedia (Pi)ATCC 25261 and 4 strains of cariogenic bacteria,Streptococcus mutans(Sm )Ingbritt,Streptococcus sanguis(Ss )34, Actinomyces viscosus(Av)19246 and Lactobacillus acidophilus(La)ATCC 4356 were used to determine the coaggregating degree of various combinations of the above bacteria by a visual assay and a turbidimetric assay. Then more than 2(or 20%) coaggregation degrees' pairs were used to investigate the inhibitory effect of lactose and arginine.Result: The coaggregation degrees of Fn-Av, Pg-Av, Fn-Sm, Fn-Ss, Fn-La and Pg-Ss pairs were higher than +2(or>20%). 4.5 mM～6.0 mM of arginine were considerably effective to most of the above pairs and the disaggregation degrees are 49%～92%. 120 mM～300 mM of lactose were significantly effective to Pg-Ss pair, the disaggregation degrees are 57%～91%. However, they are almost ineffective to Fn-G + pairs and Pg-Av pair.Conclusion: The coaagregations between perio-pathogenic and cariogenic bacteria are specific. Most of them are relatively sensitive to arginine.