MTT法检测番鸭外周血淋巴细胞转化功能的研究

Studies on MTT Colorimetric Assay for Transformation of Muscovy duck Peripheral Blood Lymphocytes

为了建立番鸭淋巴细胞转化检测的MTT法 ,筛选细胞浓度、刀豆蛋白ConA浓度和培养时间 3个参数对试验条件进行了研究。结果确定了MTT法检测番鸭淋巴细胞转化能力的最佳培养条件 ,细胞浓度 5× 1 0 6个 /mL在 2 0 μg/mLConA的RMPI 1 640完全培养基 40℃培养 60h。试验表明 。

To establish MTT assay to detect muscovy duck lymphocyte transformation ability, we chose three parameters: cell concentraion, ConA concentration and culture period. The optimum conditions were certified: cell concentraion 5×10~6/mL,ConA concentration 20 μg /mL and culture period 60 h.this study showed MTT assay could be used to detect muscovy duck cell-mediated immunity in vitro.