盐酸青藤碱对实验性自身免疫性脑脊髓炎大鼠脑内核转录因子-κB活性的作用

Sinomenine inhibits brain nuclear factor-κB activity in rat experimental autoimmune encephalomyelitis

目的 研究青藤碱的免疫抑制作用是否与核因子 κB的活性有关。方法 以实验性自身免疫性脑脊髓炎大鼠为免疫异常模型 ,观察青藤碱对其临床表现和病理特点的改变 ,检测了脑组织中核转录因子 κB的活性及血浆中皮质醇、泌乳素水平的改变。结果 青藤碱可降低EAE大鼠临床评分 ,改善病理变化 ,抑制脑组织中核转录因子 κB活性 ,增加血浆中皮质醇水平 ,降低血浆泌乳素水平。结论 青藤碱的免疫抑制作用与抑制脑组织核因子 κB活性有关 ,其对核因子 κB活性的抑制作用可能与增加皮质醇分泌。

Objective To investigate the therapeutic effects of sinomenine on the activity of brain nuclear factor-κB (NF-κB) rat in experimental autoimmune encephalomyelitis (EAE). Methods EAE rats were induced by immunization of female Wistar rats with brain and spinal cord homogenate followed by treatment with or without sinomine. The severity of EAE rats was evaluated by clinical scores and histological examination. The activity of brain NF-κB and the plasma cortisol were determined by ELISA kits. Plasma prolactin was assessed by the RIA kit. Results The brain NF-κB binding activity was increased significantly in EAE rats. The treatment of sinomenine reduced the clinical scores of EAE significantly, decreased the number of inflammatory foci in the central nervous system, and inhibited the activation of brain NF-κB in EAE rats. Besides, the level of plasma cortisol was increased and the level of plasma prolactin was decreased after the treatment of sinomenine. Conclusion Inhibiting brain NF-κB binding activity could be a mechanism of sinomenine protecting of rats from EAE, and the inhibition of NF-κB is related to the alteration of cortisol and prolactin in vivo.