噬菌体呈现的多聚多肽3A8诱导Jurkat细胞的凋亡效应

Apoptosis of Jurkat cells induced by a polypeptide 3A8 displaying on the phage surface

目的 检测噬菌体呈现的多肽 3A8诱导Fas+ Jurkat细胞的凋亡效应。方法 噬菌体表面呈现的多肽 3A8与Fas+ Jurkat细胞共孵育 ,通过Annexin V检测细胞膜磷脂磷脂酰丝氨酸的翻转 ;流式细胞仪检测经处理的Jurkat细胞DNA含量 ;透射电镜观察细胞形态是否出现典型的凋亡特征。结果 Annexin V检测到经 3A8处理的Jurkat细胞有 32 .6 4 %的细胞膜磷脂磷脂酰丝氨酸发生了翻转。处理细胞经PI染色流式细胞仪分析 ,发现 2 5 .4 1%的Jurkat细胞发生凋亡 ,明显高于未经处理的对照组。透射电镜镜下观察到凋亡细胞不同时期的形态。结论 噬菌体呈现的多肽 3A8与Jurkat细胞结合 。

Objective To test the apoptosis of Jurkat cells induced by polypeptide 3A8 on the surface of phage. Methods After incubation with the polypeptide 3A8, Jurkat cells were stained with Annexin V and propidium iodide(PI), and then analyzed by FACS. The morphological changes of the cells were observed with transmission electron microscopy. Results The phosphatidylserine on 32.64% treated Jurkat cells were externa- lized. The apoptosis rate of the treated Jurkat cells was 25.41%, which were obviously higher than that in the untreated group. Morphological changes of Jurkat cells at different times were observed. Conclusion The polypeptide 3A8 on phage could induce apoptosis of Fas + Jurkat cells.