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CPG-PNA的合成、纯化和鉴定 被引量：1

Synthesis and identification of peptide nucleic acid including CPG motif
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摘要 目的　建立肽核酸 (CPG PNA)合成、纯化、分析鉴定的方法。方法　固相肽合成标准Fmoc方法进行合成 ;中压液相层析纯化 ;反向高压液相层析进行纯度分析 ,质谱法进行分子量的鉴定。结果　经纯化 ,CPG PNA的纯度达到 95 .5 % ,分子量测定值与理论值相符。结论　合成的肽核酸与预期的一致 。 Objective To synthesize, purify, and identify peptide nucleic acid including CPG motif (CPG-PNA). Methods The CPG-PNA was synthesized by solid phase strategy, purified by fast performance liquid chromatography (FPLC), and identified by reversed phase HPLC and mass-spectrum. Results The purity of synthesized CPG-PNA was 95.5% and the molecular weight matched with theoretical values. Conclusion The synthesized CPG-PNA provides reliable basis for further researches.
作者 耿淼 吴玉章 周伟 贾正才 邹丽云
机构地区 第三军医大学基础部全军免疫学研究所
出处 《免疫学杂志》 CAS CSCD 北大核心 2005年第1期64-65,68,共3页 Immunological Journal
关键词 肽核酸 CPG 合成 鉴定 Peptide nucleic acid CPG Synthesis Identification
分类号 R392 [医药卫生—免疫学]
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参考文献10

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同被引文献42

  • 1周海清,沈鹤柏,周文骏,杨永桃.荧光探针法研究PNA-DNA双链和dsDNA结构的差异[J].上海师范大学学报（自然科学版）,2004,33(2):78-82. 被引量：2
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免疫学杂志
2005年 第1期

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