芜菁拮抗交通性污染物致小鼠遗传毒性效应的初步研究

Genetic toxicity effect of traffic pollutant on the mice and antagonistic action of Brassica rapa root juice on it.

以清洁级ICR小白鼠为实验动物,选择温州市鹿城区十里亭、火车站、南站交通岗亭为处理现场,采用小鼠骨髓嗜多染红细胞(PCE)的微核试验、小鼠精子畸形试验以及分光光度法测定小鼠肝脏过氧化氢酶(CAT)活性等实验方法,研究温州市区交通污染物对小鼠的遗传毒性及芜菁块根汁对它的拮抗效应.结果表明:经交通污染物处理的小鼠,其PCE的微核率、精子畸形率以及肝脏过氧化氢酶活性均明显高于阴性对照组;灌芜菁块根汁均可使小鼠PCE微核率、精子畸形率以及CAT活性明显下降,微核抑制率分别为62.96%、80.18%、87.36%,精子畸形抑制率分别为21.88%、45.89%、40.65%,CAT活性抑制率分别为17.27%、31.36%、37.76%.结论是交通性污染物对小鼠具有明显的遗传毒性效应;芜菁块根汁对交通性污染物引起小鼠遗传毒性效应具有显著的拮抗效应.

The study of genetic toxicity effects of traffic pollutant on the mice and antagonistic action of root juice of Brassica rapa on them in Wenzhou City was presented. By micronucleus test,sperm deformity test and CAT activity determining with spectrophotometry, the genetic toxicity was studied for mice exposed to the contaminated roads by traffic pollutant in SHI LI TING, WENZHOU RAILWAY STATION,NAN ZHAN,and antagonistic action of root juice of Brassica rapa on them were studied. The results showed that the micronucleus rates,the sperm deformity rates and the CAT activity in contaminated groups were higher than that of the control group,while the root juice of Brassica rapa can decrease the micronucleus rates,the sperm deformity rates and the CAT activity of contaminated mice by traffic pollutant,the inhibition rates of micronucleus were 6296%,80.18%,87.36%,the inhibition rates of the sperm deformity were respectively 21.88%,45.89%,40.65%,and the inhibition rates of CAT activity were 17.27%,31.36%,37.76%, respectively. The conclusion is that the traffic pollutant has the genetic toxicity effect on mice,while the root juice of Brassica rapa has the antagonistic action on them.