亚甲基蓝光化学法对全血中人免疫缺陷Ⅰ型病毒的灭活研究

Inactivation of HIV-1 in whole blood by methylene blue photochemical method

目的 观察不同浓度的亚甲基蓝 (methyleneblue ,MB)与可见光联合作用对全血中人免疫缺陷Ⅰ型病毒(HIV 1)的灭活能力。方法 以HIV 1为试验病毒 ,通过MB与可见光 ( 64 0nm)单独及联合作用于含病毒的模拟全血 ,采用MT4细胞感染法评价病毒的灭活效果。结果 当全血中MB含量分别为 5、10、15 μmol/L时 ,以 40 0 0 0Lux强度的可见光分别照射 3 0、2 0、10min ,可完全杀灭试验滴度为 10 5 78TCID50 HIV 1病毒。结论 亚甲基蓝光化学法可高效灭活全血中HIV 1病毒。

Objective To observe the ability of inactivating human immunodeficiency virus type 1 (HIV-1) in whole blood by visible light combination with methylene blue (MB) at different concentrations. Methods HIV-1 was used as the test virus. The contaminated blood was treated by MB, visible light (640 nm), combined with MB and visible light (640 nm). The test of MT4 cell infection was used to evaluate the virus inactivation efficacy. Results After being treated by MB at the concentrations of 5, 10, and 15 μmol/L, and then irradiated respectively by visible light (40 000 Lux, 640 nm) for 30, 20, and 10 min, all the added indicated virus at the titer of 10 5.78 TCID 50 of HIV-1 could be inactivated absolutely. Conclusion Methylene blue photochemical method can inactivate HIV-1 in blood effectively.