NF-κB在小鼠感染性早产发生中作用的研究

Roles of nuclear transcription factor κB in premature delivery associated with infection

目的 通过研究NF κB与感染性早产的关系 ,探索感染性早产的发病机制。方法 将 45只孕 15d的小鼠分为LPS组、LPS +PDTC(pyrrolidinedithiocarbamate)组、生理盐水组 3组 ,每组 15只 ,分别注药后观察它们的分娩时间 ,另外同样分组孕鼠 ,每组 5只 ,共 15只 ,在给予上述同样药物 16h后处死 ,以免疫组化技术检测其胎盘TNF α、IL 8水平。结果 LPS +PDTC组孕鼠的妊娠时间较LPS组明显延长 ,另 3组孕鼠检测TNF α、IL 8水平 ,发现在LPS +PDTC组中 ,其表达较LPS组明显降低。结论 NF κB活化可能是通过调控某些细胞因子的表达 ,参与感染性早产的发生。

Objective To investigate the relationship between nuclear transcription factor-κB (NF-κB) and the preterm delivery (PRD) associated with intraamniotic infection (IAI) and the related mechanisms. Methods A total of 45 pregnant mice (gestation for 15 d) were divided into three groups (n=15 in each group): lipopolysaccharide (LPS) group, pyrrolidine dithiocarbamate (PDTC) plus LPS group, and saline group. The delivery time of the mice administered intravenously with the above in each group was observed after intravenous administration. The other 15 pregnant mice were also divided into 3 groups (n=5 in each group). At 16 h after administration, these mice were sacrificed for the detection of TNF-α and IL-8 levels in the plancenta by immunohistochemistry. Results The gestation time of group administered with LPS was longer than that of the group administered with LPS plus PDTC, but the expression levels of TNF-α and IL-8 were lower than those of the group administered with LPS plus PDTC. Conclusion NF-κB may participate in PRD associated with IAI through modulating the expressions of some cytokines.