乳腺癌细胞促血管生成能力及MMP-9表达与其淋巴道转移的关系

Angiogenesis and the Expression of Matrix Metalloproteinase are Related with Lymph Node Metastasis in Breast Carcinoma

目的本研究旨在通过检测血管内皮细胞生长因子(VEGF),基质金属蛋白酶9(MMP9)在乳腺癌细胞中的表达情况及计数微血管密度(Microvasculardensity,MVD)值来探讨它们与乳腺癌浸润和转移的关系。方法采用免疫组织化学SP法检测75例乳腺浸润癌(有淋巴结转移者39例,无淋巴结转移者36例)中VEGF、MMP9、CD34的表达,并用多因素COX比例风险模型分析患者的预后。结果VEGF、MMP9和MVD在淋巴结转移组与无转移组的表达均具有显著性差异(P<0.05),与乳腺癌淋巴结转移呈正相关。MVD值高者生存时间短。结论乳腺癌淋巴道转移与VEGF、MMP9表达及MVD值有关,检测这几种蛋白表达将有助于判断乳腺癌的转移潜能及预后。

Objective To determine the expressions of vascular endothelial cell growth factor(VEGF), matrix metalloproteinase-9(MMP-9) and microvascular density(MVD) in breast carcinoma tissue and investigate their associations with invasion and metastasis.Methods The expressions of VEGF, MMP-9 and CD34 were examined in 75 cases of breast carcinoma including 39 cases with lymph node metastasis and 36 cases without lymph node metastasis by immunohistochemistry. The relationship between these expressions and prognosis was analyzed by Multivariable Cox proportional hazards model. Results The expressions of VEGF,MMP-9 and MVD in lymph node metastasis group and in no lymph node metastasis group were significantly different(P<0.05). Patients of the low MVD average group had longer survival time.Conclusion Expressions of VEGF, MMP-9 and MVD are strongly associated with lymph node metastasis of breast carcinoma. The expressions of these proteins are indicators of metastasis and predictors for prognosis of breast carcinoma.