TGF-β1、TβRⅡ及cyclinD1在大肠癌的表达及其意义

Significance of TGF-β1, TβRⅡ and cyclinD1 Expression in Colorectal Carcinoma

目的探讨TGFβ1、TβRⅡ及cyclinD1在大肠癌发生发展过程中的作用。方法采用免疫组化SP法从蛋白水平分析TGFβ1、TβRⅡ、cyclinD1在大肠腺瘤及大肠癌组织中的表达情况。结果TGFβ1蛋白阳性表达率在腺瘤组织为28.6%,大肠癌组织中为57.1%(P<0.05);TβRⅡ在大肠癌组织中的表达率为51.8%,低于腺瘤组织(P<0.01);TGFβ1、TβRⅡ表达的改变与大肠癌浸润深度、淋巴结转移有关;TβRⅡ和cyclinD1的表达呈负相关(P<0.05)。结论TGFβ1的过表达与大肠癌侵袭转移演进密切相关;TβRⅡ的失表达不仅有助于提高细胞对TGFβ1的生长抵抗,也增强了大肠癌的侵袭性;cyclinD1在TGFβ1的生长抵抗中可能发挥了某些作用。

Objective To investigate the role of TGF-β1, TβRⅡ,cyclinD1 expression in colorectal carcinoma.Methods SP immunostaining technique was used to examine the expression of TGF-β1, TβRⅡ and cyclinD1 in colorectal adenomas and carcinomas. Results TGF-β1 was expressed positively in 28.6% colorectal adenoma tissues while ~57.1% in carcinoma(P<~0.05 ); TβRⅡ was expressed positively ~51.8% in colorectal carcinoma tissues which was lower than adenoma tissues(P<~0.01 );The expression of TGF-β1、TβRⅡ in colorectal tissues was associated with colorectal carcinoma infiltration depth, lymph node metastasis; A negative correlation was observed between the expression of TβRⅡ and that of cyclinD1(P<~0.05 ). Conclusion The overexpression of TGF-β1 was highly related to the invasion or metastasis of colorectal carcinoma; Decreased expression of TβRⅡ was helpful not only to enhance cell growth resistance to TGF-β1 but increase the invasion of colorectal carcinoma; cyclinD1 may played a role in the resistance of TGF-β1.