呼吸系统疾病患者血清PTPr含量分析

Quantification of O-Phospho-L-tyrosine Protein(PTPr) in the Sera of Respiratory Patients

目的研究呼吸系统疾病患者血清PTPr含量及其意义。方法用ELISA法测患者血清PTPr,然后分组统计分析。结果48例肺癌PTPr含量(36.4±27.8)ng/ml,阳性(>30ng/ml)32例(66.7%),十分显著高于以下各组(P<0.001);肺结核病48例PTPr含量(7.9±14.1)ng/ml,阳性4例(8.3%);肺炎56例PTPr含量(11.6±16.6)ng/ml,阳性4例(7.1%);其他疾病54例PTPr含量(8.1±15.6)ng/ml,阳性4例(7.4%)。结论肺癌患者血清PTPr含量显著增高。提示肺癌发生发展中信号传导分子活化增强;测定PTPr对肺癌诊断可能具有临床价值。

Objective The quantities of serum O-phospho-L-tyrosine protein (PTPr) in groups of respiratory patients and its value were studied.Methods An ELISA was applied to detect serum PTPr of the groups of patients, and then the results of each group were statistically analyzed and compared. Results The serum PTPr in lung cancer group was ^(36.4 ±^27.8) ng/ml; 36(~66.7% ) in the 48 cases were positive (PTPr>30ng/ml); and the both were significantly higher than those in the following groups (P<~0.001 ). The PTPr amounts and positives in pneumo-tuberculosis, pneumonia and other respiratory diseases groups were respectively (~7.9 ±~14.1 )ng/ml and ~8.3% (4/48), ^(11.6 ± ^16.6 )ng/ml and ~7.1% (4/56), and (~8.1 ±~15.6 )ng/ml and ~7.4% (4/54). Conclusion In lung cancer patients the serum PTPr were increased evidently; it suggested that the activation of signaling molecules be enhanced in lung cancer cell generation and growth, and assaying PTPr may be of value in lung cancer diagnosis.