小剂量顺铂联合^(32)P玻璃微球对小鼠S180瘤细胞的细胞毒作用

Cellular Toxic Effect of Low Dose Cisplatin Combined with Interstitial Injection of ^(32)P Glass Microspheres on Mouse Solid Tumor S180

【目的】研究腹腔注射小剂量顺铂联合瘤内注射32P玻璃微球对小鼠S180实体瘤细胞的细胞毒作用,通过透射电镜观察比较不同治疗对肿瘤细胞的超微结构影响。【方法】将实验动物荷S180瘤小鼠28只随机分为4组,各组7只。4组小鼠随机定为空白对照组、单纯顺铂组、单纯32P玻璃微球间质注射组和顺铂联合32P玻璃微球间质注射组,治疗两周后取出肿瘤称瘤体质量,计算各组抑瘤率。分别取各组肿瘤组织行透射电镜观察,比较不同治疗对肿瘤细胞超微结构的影响。【结果】单纯顺铂组、单纯32P玻璃微球间质注射组及顺铂联合32P玻璃微球间质注射组抑瘤率分别为47%、40%、76%。小剂量顺铂联合32P玻璃微球间质注射组与两单独治疗组抑瘤率的差异有统计学意义(P﹤0.01)。电镜观察联合组细胞死亡率达75%,绝大部分肿瘤细胞消失,呈大片坏死结构。【结论】腹腔注射小剂量顺铂联合32P玻璃微球间质注射内放射治疗对小鼠S180实体瘤细胞的细胞毒作用较单独应用有明显增强。

To investigate the cellular toxic effect of a combination of low dose cisplatin and 32P glass microsphere (32P-GMS) on mouse solid tumor S180. Twenty-eight mouse with solid tumor S180 were randomly divided into four groups (control group, cisplatin group, 32P-GMS group, combination of cisplatin and 32P-GMS group), and 7 for each group. After 2 weeks of treatment the weight of tumor was measured separately. The death rate of tumor cells and the inhibition rate of tumor were calculated respectively. The ultrastructural changes of specimens were observed by transmission electron microscopy. The inhibition rates in the three treatment groups were 47%, 40%, and 76%, respectively. There were significant differences in the inhibition rates between combination of cisplatin and 32P-GMS group and other groups (P﹤0.01). The death rate of tumor cells was 75% in the combination therapy group, which was significantly higher than those in other groups by observing under the electronic microscope. Most of tumor cells were disappeared which displayed a large area of necrosis structures. [Conclusion] The combination using of low dose cisplatin interstitial injection of 32P-GMS is able to enhance the cellular toxic effect on mouse solid tumor S180 comparing to single using of cisplatin or 32P-GMS .