多噻烷诱导芳香烃受体作用研究

A Study on Poisoning Mechanism of Polythiacycaoalkanes (PCTA)

目的 :通过特殊的细胞株研究多噻烷有无诱导芳香烃受体的作用。方法 :用化学激活荧光素酶表达方法 ,离体测试多噻烷诱导完整细胞中芳香烃受体依赖的基因表达。结果 :多噻烷各浓度对人肝细胞瘤细胞有毒性 ,抑制细胞生长 ;对小鼠肝细胞瘤细胞基本无毒 ;各浓度在人和小鼠肝细胞瘤细胞中所致荧光素酶活性均未明显高于阴性对照组 (DMSO)。结论 :多噻烷对人和小鼠肝细胞瘤细胞无诱导芳香烃受体的作用。

Objective: To investigate if PCTA have effect of inducing aromatic hydrocarbon receptor (AhR). Methods: The in vitro chemical activated luciferase expression (CALUX) assay was adopted, and the human and mouse hepatoma cell lines were used. Results: The tested concentrations of PCTA were toxic to human hepatoma TV101L cells, and inhibited the growth of the cells, but were basically non toxic to the mouse hepatoma Hepal.12cR cells. The luciferase activities of PCTA group were not significantly higher than that of control group (DMSO) tested in both TV101L and Hepa1 12cR cell lines. Conclusion: PCTA has no inducing effect of AhR dependent expression in both human TV101L and mouse Hepal.12cR cell lines. [