基因探针和PCR方法在菌痢流行病学研究中的应用

Application of gene probe and PCR in the epidemiological studies of Shigellosis

目的 探讨基因探针和PCR方法在F2a志贺氏菌痢暴发的流行病学研究中的意义。方法 对 4 3株自患者粪便和食物中分离到的F2a志贺氏菌进行 16srRNA、ipaH基因Southern杂交 ,随机PCR和set1/set2毒力基因PCR分析。 结果 随机PCR、ipaH基因Southern杂交将 4 3株F2a志贺氏菌分为两个不同的基因型 ,set1/set2毒力基因PCR分为三个不同的基因型 ,16srRNA基因Southern杂交均为同一基因型。结论 基因分型方法能更准确、深入地揭示菌痢暴发过程中各分离株之间的流行病学联系。其中set1/set2毒力基因PCR方法具有较高的分辨率 ,在F2a志贺氏菌的分子流行病学研究中具有重要的作用。

To investigate the significance of gene probe and PCR methods in the epidemiological studies of Shigellosis, 16srRNA and ipaH gene Southern hybridization, RAPD PCR and Set1/set2 PCR were performed in 43 strains of Shigella F2a isolated from stools of patients and from foods. It was found that these 43 isolates were subtyped into two genotypes by means of RAPD PCR and ipaH gene Southern hybridization, but they were subtyped to three genotypes with Set1/set2 PCR; on the other hand, they could not be subtyped by 16srRNA gene Southern hybridization. It concludes that these genotypic methods of investigation can explore the epidemiological relationship of isolates in a Shigella F2a outbreak accurately and profoundly, in which Set1/set2 PCR has higher resolving power, and is important in the molecular epidemiology of Shigellosis outbreak, whereas 16srRNA gene Southern hybridization has no resolving power, and is not suitable for the proper genotyping of Shigella F2a.