玉竹提取物B对CEM的抑制作用

The Inhibitory Effect of EB-PAOA on CEM

目的 研究玉竹提取物B (ExtractionBofpoly -gonatomodoratum ,EB -PAOA)对CEM和人类正常T细胞作用。方法 以不同浓度的EB -PAOA处理CEM细胞和正常人T细胞 ,采用MTT法测EB -PAOA对CEM及人类T细胞增殖的抑制作用 ,采用电镜检测CEM细胞形态学的变化 ,采用流式细胞仪及琼脂糖电泳检测DNA的变化 ,观察CEM在EB -PAOA的作用下发生凋亡的情况。结果 最低浓度 (1∶4 0 0 0 /ml)对CEM的增殖抑制作用较小 ,随浓度的增大抑制作用逐渐增强 ,最高浓度的抑制作用最强 (P <0 0 1) ;可促进CEM细胞凋亡 ;EB -PAOA对人的正常T淋巴细胞的增殖没有影响 (P >0 0 5 )。结论 EB -PAOA可以抑制CEM的增殖 ,其抑制作用随浓度增加逐渐增强 ;并能诱导CEM细胞凋亡 ;EB -PAOA对人类T淋巴细胞的增殖没有影响。

Objective To research the inhibitory action of EB-PAOA on the proliferation of CEM cells.Methods To assay for the proliferation of CEM and human T cells with MTT assay, to observe the apoptosis with electron microscope, agarose electrophoresis and flow cytometer.Results 1∶4000ml^-1had little effect on CEM, with concentration increasing, inhibitory action enforced, 1∶500ml^-1 had the greatest action (P<0. 01). It could induce apoptosis. EB-PAOA had no affection on human T cells (P>0.05). Conclusions EB-PAOA could inhibit the proliferation of CEM and induce apoptosis. It had no effect on human T cells.