MTT比色分析法检测卵巢癌药物敏感性

Detection of chemosensitivity of ovarian cancer with MTT colorimetric assay

目的探讨四氮唑蓝比色分析法(MTT法)用于指导临床个体化化疗药物选择的实用性,为卵巢癌临床化疗用药提供参考。方法采用常用的12种化疗药物对65例卵巢癌进行MTT法药物敏感试验。结果不同类型的卵巢癌及同一类型的不同个体对化疗药物的敏感性差异较大。卵巢癌对紫杉醇、表阿霉素、卡铂和顺铂中度敏感,平均抑制率分别为(67.3±4.6)%、(54.5±4.8)%、(52.0±4.3)%和(50.8±4.5)%;对鬼臼乙叉甙、烃基喜树碱和长春地新低度敏感,平均抑制率分别为(39.6±3.8)%、(39.3±4.3)%和(30.7±2.6)%;而对丝裂霉素C、噻替哌、5氟尿嘧啶、长春新碱和氨甲喋呤则耐药,平均抑制率均小于30%。结论卵巢癌化疗敏感性个体差异较大。MTT法是一种简便而快速的肿瘤体外药物敏感实验方法,可为卵巢癌临床个体化化疗方案的选择提供客观依据。

Objective: To assess the practicability of in vitro chemosensitivity testing in ovarian cancer using MTT colorimetric assay, and to help clinical doctors choose more effective chemotherapeutic agents. Methods:Chemosensitivity of 65 ovarian cancer samples to 12 drugs were detected in vitro with MTT assay. Results:There was a great difference in sensitivity to anticancer agents between different kinds of ovarian cancer and between patients with same kind of ovarian cancer. Ovarian cancer cells had great sensitivity to Paclitaxel,Epirubicin,carboplatin, and Cisplatin at (67.3±4.6)%,(54.5±4.8)%,(52.0±4.3)%,and (50.8±4.5)% of average inhibition rate respectively. Low sensitivity was shown to Etoposide,Hydrocarbonyl Camptothecin, and Vindesine at (39.6±3.8)%,(39.3±4.3)%,and (30.7±2.6)% of average inhibition rate respectively. There was drug resistance to mitomycin C,Thiotepa,5-Fluorouracil,Vincristine, and Methotrexate at average inhibition rate less than 30%. Conclusion:There was a great difference in sensitivity to anticancer agents between different patients with ovarian cancer. The MTT assay is a simple technique, rapid for testing of chemosensitivity, and helpful to compose individual chemotherapeutic scheme for patients.