思他宁对人胰腺癌细胞株ASPC-1的生长调控

Growth modulation of stilamin on human pancreas carcinoma cell line ASPC-1

目的观察思他宁对人胰腺癌细胞ASPC1的生长作用。方法细胞培养,分别加入不同浓度的思他宁。应用MTT法来观察细胞增殖程度;放免法测定细胞内cAMP含量;荧光法测定[Ca2+]i。结果不同浓度的思他宁(10-11～10-6mol/L)均能有效地抑制ASPC1的生长,能抑制ASPC1细胞内cAMP生成和细胞内钙离子水平,cAMP生成量和细胞内钙离子水平与思他宁浓度呈负相关。结论思他宁能抑制ASPC1细胞的生长,经过特异性受体调节。

Objective: To investigate the effect of stilamin on the growth of human pancreas carcinoma cell strain ASPC-1. Methods:The ASPC-1 cells were cultivated with different concentrations of stilamin. Proliferation of cells was measured by MTT method.Intracellular levels of adenosine monophosphate was determined with radioimmunoassay . Intracellular levels of calcium was measured with flurorescence spectrophotometer. Results:Stilamin inhibited the proliferation of ASPC-1 in a concentration dependent manner(P<0.01). Stilamin inhibited production of intracellular cAMP, and Ca 2+ in ASPC-1 cells. The amount of production was negatively correlated with concentration of stilamin(P<0.01).Conclusion:The inhibitive effect of stilamin on proliferation of ASPC-1 might be mediated by specific receptor.