双歧杆菌的完整肽聚糖对巨噬细胞产生IL-18的影响

The influence of whole peptidoglycan of Bifidobacterium on interleukin 18 produced by macrophages

目的 探讨分叉双歧杆菌的完整肽聚糖对巨噬细胞功能的调节作用。方法 以完整肽聚糖注射于小鼠腹腔 ,用ELISA法测定小鼠腹腔巨噬细胞产生的IL 18的含量。结果 完整肽聚糖注射组小鼠腹腔巨噬细胞产生的IL 18的含量显著高于对照组 (P <0 0 1)。结论 分叉双歧杆菌的完整肽聚糖能激活巨噬细胞 ,并使之分泌多量的IL 18。

Objective To explore the adjustment of whole peptidoglycans of Bifidobacteria to macrophage functions.Methods The level of interleukin 1 and tumor necrosis factor α and nitric oxide(NO) produced by peritoneal macrophages of nude mice was detected by employing mouse thymocyte proliferation method killing L929 cell method in vitro and Griess reagent,after whole peptidoglycans were injected into the nude mice peritoneally.Simultaneously,tumoricidal activity of the macrophages in vitro was observed.Results The content of interleukin 1 and tumor necrosis factor α and nitric oxide(NO) secreted from peritoneal macrophages of nude mice was significantly higher in the whole peptidoglycan group when compared with the control group.Futhermore,the tumoricidal activity of the macrophages in vitro was markedly promoted( P <0.01).Conclusion Whole peptidoglycans of Bifidobacterium bifidum could activated macrophages to secrete a lot of cytotoxicity effectors,and elevate its tumoricidal activity.