坛紫菜叶状体体细胞对抗生素敏感性的研究

Antibiotics sensitivities of somatic cells of Porphyra haitanensis thallus

坛紫菜(Porphyrahaitanensis)叶状体体细胞对氨苄青霉素有很强的耐受性,但氨苄青霉素对体细胞分裂、分化具有明显的抑制作用,导致更多的体细胞分化形成细胞团,并能抑制叶状体假根的形成.坛紫菜叶状体体细胞对氯霉素敏感,100μg/cm3和800μg/cm3组的体细胞分别于培养的12、10d全部死亡.本试验结果初步表明,氯霉素可作为坛紫菜基因工程的有效选择压力.

It is shown that the tolerance of somatic cells of Porphyra haitanensis thallus to ampicillin is up to 7 000μg/cm^3 in 16 days; but the divisionand differentiation are inhibited obviously, while the cell aggregation is elevated and the formation of rhizoid of thallus inhibited. On the other hand,the somatic cells of P. haitanensis thallus is sensitive to chloromycetin. The somatic cellsin 100μg/cm^3 and 800μg/cm^3 groups are all dead in 12 and 10 days respectively.Our conclution is that the chloromycetin can be used as an effective selective pressure in the genetic engineering of P. haitanensis.