正常育龄妇女及孕妇巨细胞病毒IgG和IgM的检测

SEROLOGICAL DETECTION OF CMV-lgG AND lgM ANTIBODIES IN BEARING-AGE AND PREGNANCY WOMEN

采用间接免疫荧光法(IIF)对18～45岁的育龄妇女191人及各期妊娠妇女237人进行巨细胞病毒(CMV)IgG 和 IgM 检测,特异性 CMV-IgM 检测采用细胞核涂片法,结果育龄妇女和妊娠妇女的 CMV-IgG 阳性率分别是97.9%和99.6%,几何平均滴度(GMT)分别为1∶177.9和1∶184。特异性 CMV-IgM 阳性者(>1∶10)有19例,其中妊娠妇女14例(5.9%),育龄妇女5例(2.6%)。实验证明用细胞核涂片法检测特异性 CMV-IgM 具有快速、特异和敏感的优点。

We report the results by applying the indirect immuneflourest assay to the detecting of CMV specific-IgG and IgM antibodies in the sera from 191 women of bearing age and 237 during pregnancy.Positivety rate for CMV-IgG were 97.6% and 99.6% in the sera of both groups respectively.GMT were 1:177.9 aud 1:184 respectively.The CMV-specific IgM antibody was measured in sera of all women by blood smear nuclear technigue of using nuclei of virus-infected fibroblast cells as antigens.The result was that 19 women were found positive,including 5(2.6%) in the bearin-gage group and 14(5.9%)in the pregnancy group.