胃癌浸润转移——整合蛋白、Ⅳ型胶原酶及细胞外基质的作用

Invasion and Metastasis in Gastric Carcinoma——Influence of Integrin, Type Ⅳ Coilagenase and Extracellular Matrix

用免疫组织化学方法研究76例胃癌标本中整合蛋白α6亚基、层粘连蛋白(Ln)、Ⅳ型胶原(Col Ⅳ)及Ⅳ型胶原酶(Col Ⅳ酶)及ras P21的表达以探索胃癌浸润转移的机制,结果发现:α6及Col Ⅳ酶在胃癌细胞的表达增强,α6与Ln在基底膜上呈协调表达,而Col Ⅳ酶与Col Ⅳ在基底膜上的表达呈反比。提示α6(代表Ln受体)及其配体(Ln)通过细胞—细胞外基质的相互作用影响胃癌的浸润性生长;而Col Ⅳ酶通过分解Gol Ⅳ而促进胃癌浸润转移。且与ras P21的表达呈正相关,可能是ras癌基因活化而参与调控胃癌浸润转移表型的途径之一。它们可作为反映浸润转移性状的标志。

In exploring the mechanism of invasion and meteastasis, the expression of integrin alpha-6 subtmit, laminin, type Ⅳ collagenase, type Ⅳ collagen, and ms P21 wasv studied immunohistochemically in 76 cases of gastric carcinoma specimens. Results showed that the expresssion of α6 subunit (laminin receptor) was markedly enhanced on the surface of gastric cancer cells, and exhibited coordinate expression with its legand, laminin, on the basement membrane. These indicated that d6 and laminin, through their cell-ECM interactions may contribute to the invasive growth. Intensc expression of type Ⅳ collagenase was frequetntl y found in gastric carcinoma cells, especially in the infiltrative type and in those with lymph node metastasis. Thus, type Ⅳ collagenase may be useful in predicting the invasion and metastasis of cancer cells, and a positive correlation was found between the expression of type Ⅳ collagenase and ms P21. These suggested that there may be a genetic linkage between type Ⅳ collagenase expression and the invasion and metastasis pheynotype through ms oncogene activation. And these molecules might be used as effective biological markers for the malignant phenotype of gastric carcinoma.