利用重新设计的引物对三个Y-STR基因座进行复合扩增

A Multiplex for Simultaneous Amplification of Three Y-STR Loci Using Modified Primers

目的 改良一种同步检测 Y- STR基因座的方法。方法 采用重新设计的 Y- STR基因座引物 ,用优化的复合扩增技术同时扩增 3个 Y - STR基因座 (DYS390、DYS391、DYS393)。结果 成功地同步扩增出 3个 Y-STR基因座 ,将其中 DYS391基因座的片段大小由 2 79～ 2 87bp缩短至 14 2～ 15 0 bp,同时还提高了 DYS393基因座的男性特异性。结论 利用重新设计的 Y - STR基因座引物进行复合扩增 ,为快速检测多个基因座提供了有效的途径 。

Objective To develop a multiplex method for simultaneous detection of Y-STR loci. Methods The primers of Y-STR loci were devised for use in the simultaneous amplification of three Y-STR loci(DYS390,DYS391,DYS393). Results Simultaneous amplification of the three Y-STR loci was successfully performed using the modified primers in which the fragment length of DYS391 was shortened from 279-287 bp to 142-150 bp, and at the same time, the virile specificity of DYS393 was enhanced. Conclusion The use of new-devised primers of Y-STR loci is a valid approach to rapid detection of multiple Y-STRs loci; it is worthy to be recommended in the field of forensic science.