PDGF-B在闭合性脑损伤组织中的表达

Expression of PDGF-B after Fluid Percussion Brain Injury in Rats

目的 了解闭合性脑损伤后 PDGF- B的表达变化。方法 制备液压冲击脑损伤大鼠模型 ,应用免疫组化技术观察大鼠脑损伤后不同时间 PDGF- B的表达情况 ,并应用图象分析仪进行分析。结果 脑损伤后 1hPDGF- B表达上调 ,4～ 7d达高峰 ,14 d时仍高于对照组。结论 液压冲击脑损伤后 PDGF- B表达上调 ,且呈一定的时序性变化规律 。

Objective To elucidate the changes of PDGF-B chain in the brain injured by fluid percussion. Methods The expression of PDGF-B protein was studied by immunohistochemistry and the results were assessed by image analysis system. Results Enhanced expression of PDGF-B protein induced by fluid percussion injury was seen at 1 h, and its marked up-regulation was noted at 4-7 days, and then the expression became decreased but it remained above the control levels till 14 days after injury. Conclusion Fluid percussion brain injury induced expression of PDGF-B. The regular change pattern of PDGF-B level in time course seems to be of value in estimating the age of brain injury.