益生注射液对肾脏热缺血再灌注氧化应激损伤的作用研究

Effects of YM Injection on Oxidative Stress Injury in Renal Warm Ischemia/Reperfusion

目的 观察益生注射液 (YM)能否减轻小鼠肾脏热缺血再灌注 (I/ R)损伤。方法 C5 7BL/ 6小鼠肾脏热缺血 5 0 m in,再灌注 2 4 h取血和组织。术前 12 h和 30 m in分别腹腔给予不同剂量的 YM。观察 YM对 I/ R引起的氧化应激、炎症介质的影响。结果 I/ R导致小鼠肾脏严重损伤。给予 YM(5、15、2 5 m g/ kg) ,肾脏丙二醛(MDA)水平分别降低 16 .6 %、2 5 .0 %和 35 .5 % ,超氧化物歧化酶 (SOD)活性分别增加约 38.7%、4 8.3%和76 .1%。YM(2 5 m g/ kg)可明显抑制 ICAM- 1的表达上调。结论 YM至少部分通过降低活性氧的产生、减少粘附分子的表达来减轻小鼠肾脏 I/

Objective This study was undertaken to determine whether YM injection would attenuate warm I/R injury in mice. Methods Renal warm I/R was induced in C57BL/6 males, and the mice were sacrificed 24 hours post-reperfusion. YM was given intraperitoneally 12 hours and 30 min before I/R. The effects of YM on oxidative stress and proinflammatory mediators caused by renal I/R injury were assayed. Results Renal I/R caused severe injuries in the kidneys of mice. In the presence of YM at doses of 5, 15 and 25 mg/kg, the kidney malondialdehyde (MDA) content reduced by 16.6%, 25.0% and 35.5% respectively, and the kidney superoxide dismutase(SOD) activities were elevated by about 38.7%, 48.3% and 76.1% respectively. ICAM-1 expression was dramatically suppressed in the presence of YM (25 mg/kg). Conclusion YM attenuates the renal warm I/R injury at least partially via decreasing the neutrophils infiltration and suppressing the over-expression of adhesion molecules.