转基因插入对水稻花粉活力和杂交结实的影响

Effects of transgenes insertion on pollen vigor and hybrid seed set of rice.

以花粉活力和杂交结实率评价转基因插入对水稻品种异交潜力的影响.选用含bar、crylAb、BADH和Xa21等4种基因的5份转基因水稻品系研究转基因插入对转基因受体品种花粉离体萌发率的影响.结果表明,不同受体品种花粉离体萌发率差异显著;转基因水稻花粉离体萌发率在0416～0584间,与受体品种(0400～0574)相近;转基因品种与相应受体品种间花粉离体萌发率差异不显著.对所选配的26个人工杂交组合杂种结实调查表明,bar、crylAb基因的插入对受体品种杂交结实率影响显著,而Xa21基因的插入对受体品种杂交结实率影响较小;转基因水稻品种(花粉供体)与非转基因水稻品种的杂交结实率变幅为0056～0413,在受体品种(花粉供体)与非转基因水稻品种的杂交结实率范围(0052～0417)之内.本研究花粉离体萌发和杂交结实结果表明转基因的插入对水稻品种异交潜力的影响甚微.

In this paper,the effects of transgenes insertion on the outcross potentiality of rice varieties were assessed by pollen vigor and hybrid seed set.The in vitro pollen germination rates of five transgenic rice lines transformed respectively with bar,crylAb,BADH and Xa21 gene were investigated,and compared with their relative receptor rice varieties.The results showed that there existed a significant difference in in vitro pollen germination rates between receptor rice varieties,but no significant difference was found between transgenic rice varieties and their relative receptors.The in vitro pollen germination rate for transgenic rice varieties varied from 0.416 to 0.584,similar to that of their relative receptors(0.400～0.574).Investigation on the hybrid seed set of 26 hand-crosses showed that the inserted bar or crylAb gene had a significant effect on the hybrid seed set of receptors,while the effect of Xa21 gene was smaller.The hybrid seed set rate of non-transgenic rice in crosses with transgenic rice(pollen donor)was from 0.056 to 0.413,not different from that in crosses with their relative receptors(0.052～0.417).It's suggested that transgenes insertion had little effect on the outcross potentiality of rice varieties.